| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1221 | Peribacillus simplex | CCATGACAGAGGCAGCCAA | TTTCGCACGGTCCGCTTT | 60.00 | 60.59 | 220 | 50 |
100.00%
|
100.00%
|
| Primer 1222 | Peribacillus simplex | CCGGTCAGCCAGATGGTTT | ATTCGCGAATGGCACCCT | 60.00 | 59.73 | 151 | 50 |
100.00%
|
100.00%
|
| Primer 1223 | Peribacillus simplex | TTGCTGCGGTAAAGCGGA | AGCTCGACCTGGACCTCAT | 59.97 | 60.00 | 159 | 50 |
100.00%
|
100.00%
|
| Primer 1224 | Peribacillus simplex | AGCGCTCAAACCAGGCAA | TTCGGTGAGGACGTATGGC | 60.20 | 59.49 | 261 | 50 |
100.00%
|
100.00%
|
| Primer 1225 | Peribacillus simplex | GCACAAGCCGAAGCCTTT | TGGTTCCATGCAATCCGGT | 58.96 | 59.62 | 232 | 50 |
100.00%
|
100.00%
|
| Primer 1226 | Peribacillus simplex | GCACAAGCCGAAGCCTTT | GGTTCCATGCAATCCGGTG | 58.96 | 59.19 | 231 | 50 |
100.00%
|
100.00%
|
| Primer 1227 | Peribacillus simplex | CCGGATTTGTGATGCACGG | ATCGCCAACAATGCCTGC | 59.57 | 59.11 | 214 | 50 |
100.00%
|
100.00%
|
| Primer 1228 | Peribacillus simplex | TGGTTCAACCGGTGCTTGA | TGAGCATGACGGCTGCAA | 59.78 | 59.97 | 288 | 50 |
100.00%
|
100.00%
|
| Primer 1229 | Peribacillus simplex | TTGTCCGGACGATGCCAA | ATCACGGACCCCTCCATCA | 59.26 | 60.00 | 236 | 50 |
100.00%
|
100.00%
|
| Primer 1230 | Peribacillus simplex | TTGAAGACCTGGTGCGGTG | CCCTGTCCGACCTTCGTTT | 60.23 | 59.63 | 275 | 50 |
100.00%
|
100.00%
|